Plasmid

Part:BBa_K5048024:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid AQ_6xhis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5665
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5665
    Illegal NotI site found at 5690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5665
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5659
    Illegal XhoI site found at 5699
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5665
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5665
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
    Illegal AgeI site found at 5409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

We add enterokinase site between Lpp'OmpA and small peptides in order to verify the enzymatic cleavage.



Source

The insertion fragment is complete synthesis . The backbone is purchased from Genscript.


References